Inhibition of nucleoside-binding sites by nucleoside analogues in Escherichia coli

Inhibition of nucleoside-binding sites by nucleoside analogues in Escherichia coli.

SUMMARY Nucleoside analogues modified in heteroeyclic bases or sugar moieties were screened for their ability of counteracting the bacteriostatic effect of showdomycin in E. colio Pyrimidine ribonucleosides or 2-deoxyribonucleosides substituted at the position 5 wijh halogen^atom, methyl or hydroxyl group, 2-fluoro-, 2-chloro-2-deoxyuridme and its 5-methyl homologue, N^-dimethylcytidine and N*-...

Inducible and constitutive nucleoside-binding sites in Escherichia coli: differential inhibition by nucleoside analogues.

Constitutive and inducible nucleoside-binding sites on the surface of the cells of E. coli B have different patterns of inhibition by nucleoside analogues. 1(beta-D-Ribofuranosyl)-4-aminopyrimidine-6-one and isoguanosine preferentially inhibit the inducible binding sites while showdomycin and N(4)-dimethylcytidine interfere more strongly with the constitutive function.

Chemical Synthesis of Nucleoside Analogues Chemical Synthesis of Nucleoside Analogues

Coupled nucleoside phosphorylase reactions in Escherichia coli.

Nucleoside diphosphate kinase from Escherichia coli.

Nucleoside diphosphate (NDP) kinase from Escherichia coli was purified to homogeneity and was crystallized. Gel filtration analysis of the purified enzyme indicated that it forms a tetramer. The enzyme was phosphorylated with [gamma-32P]ATP, and the pH stability profile of the phosphoenzyme indicated that two different amino acid residues were phosphorylated. Both a histidine residue and serine...